Superior Broth™

Superior Broth™ is a proprietary medium formulation developed by AthenaES™ for improved expression of recombinant proteins in E. coli. The medium is a complex, rich formulation which supplies a nitrogen source, vitamins, and moderate glucose levels. The medium is buffered at pH 7.2±0.2 to prevent acidification of the medium during metabolism of the glucose. For expression of recombinant proteins, induction should be done at cell densities twice that of LB Broth. Cell yields are typically about 3 to 4 times that of LB Broth in shake flask cultures depending on the characteristics of the strain. Plasmid copy numbers are the highest among the AthenaES family of media. This medium is an excellent choice for large-scale plasmid preparations. Biomass yields will depend on the process employed and the strain.