SUMO-QAPTURE-T^® kit

• Fast, simple, and reliable assay
• High specificity, high through-put capacity
• Amenable to analysis via Western blotting or proteomic methods

The SUMO-QAPTURE-T^® kit is an efficient tool for the selective isolation of SUMOylated proteins. The kit facilitates the affinity purification of SUMOylated proteins from cell extracts and tissue lysates using a high-binding SIM-containing affinity matrix. Captured proteins are eluted under denaturing conditions followed by analysis by Western blotting, using the SUMO antibodies provided or antibodies to specific proteins of interest, or potential substrate identification by proteomic methods. SUMO-conjugate-containing samples are prepared in native form in the presence of protease inhibitors to prevent loss through the action of deSUMOylating enzymes. Optimization of binding permits complete isolation of the full range of SUMO-protein conjugates from a specific lysate.