Succinate Assay

Succinate is an important metabolite in the tricarboxylic acid cycle. Succinate has also emerged as a signaling molecule in various cellular processes, including inflammation, hypoxia responses, and regulation of gene expression. In pathological conditions such as ischemia, it accumulates, impairing mitochondrial function and promoting oxidative stress and tissue damage. AkrivisBio’s Succinate assay is a simple, sensitive method for the accurate determination of succinate in a variety of samples at levels below 25 μM. In the assay, succinate is converted to succinyl CoA with the formation of an intermediate, which then undergoes a series of reactions to generate NADH. The NADH formed then reduces nearly colorless tetrazolium to a highly colored formazan measured at OD 450 nm.