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Substance P ELISA kit

ADI-900-018A

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Highly sensitive Substance P ELISA kit enabling detection of Substance P in plasma, serum, saliva, urine, and supernatants in just 3 hours.

Highly sensitive assay, measuring as little as 5.3 pg/ml of Substance P
Widely cited in numerous peer reviewed publications
High throughput fomat, assay up to 38 samples in duplicate per 96 well kit
Fully quantitative results that surpass semi-quantitative Western blot analysis

The Substance P ELISA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. This kit utilized low sample volume and provides the ability to assay up to 38 samples in duplicate per 96 well kit.

Substance P is an undecapeptide hormone (RPKPQQFFGLM) that mediates a number of biological activities including nausea, anxiety, pain, and vasodilation. It is a member of the tachykinin family of neuropeptides, and is widely distributed in the peripheral and central nervous systems of vertebrates.

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Product Details

Application	Colorimetric detection, ELISA
Application Notes	For the quantitative determination of Substance P in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample type includes tissue.
Assay Time	3 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Sensitivity	5.3 pg/ml (range 9.76 – 10,000 pg/ml)
Species Reactivity	Species independent
UniProt ID	P20366
Wavelength	405 nm

Handling & Storage

Use/Stability	Store components at temperatures indicated on the component labeling. The Antibody, Conjugate and Standard are stored at -20°C and the remaining components are stored at 4°C. For convenience upon receipt, the unopened kit containing all components can be stored at -20°C and are stable at that temperature until the kit’s expiration date. After first thaw, store components at temperature indicated on the labeling as indicated above. Freeze-thaw cycles should be limited for optimal kit performance. Please see Reagent Preparation section in the product manual for more detailed component handling information.
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Use/Stability

Store components at temperatures indicated on the component labeling. The Antibody, Conjugate and Standard are stored at -20°C and the remaining components are stored at 4°C. For convenience upon receipt, the unopened kit containing all components can be stored at -20°C and are stable at that temperature until the kit’s expiration date. After first thaw, store components at temperature indicated on the labeling as indicated above. Freeze-thaw cycles should be limited for optimal kit performance. Please see Reagent Preparation section in the product manual for more detailed component handling information.

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Regulatory Status	RUO – Research Use Only

Comparison of plasma substance P concentrations in the blood of healthy male and female German Simmental calves: A. Landinger, et al.; BMC Vet. Res. 20, 226 (2024), Application(s): Bovine plasma, Abstract — Full Text
Lipolysis inhibition as a treatment of clinical ketosis in dairy cows: A randomized clinical trial: Chirivi, M., Cortés-Beltrán, D., et al.; J. Dairy Sci. 106, 9514 (2023), Abstract
Evaluation of Substance P concentrations in the blood plasma of jugular and tail vein of healthy German Simmental cows: A. Landinger, et al.; BMC Vet. Res. 19, 218 (2023), Application(s): Bovine plasma, Abstract — Full Text
Functional validation of co-culture model of human keratinocytes and neuronal cell line for sensitive skin by using transient receptor potential channel vanilloid subfamily member 1 antagonist: S.M. Shin, et al.; Skin Res. Technol. 29, e13275 (2023), Abstract
Evaluation of Substance P as a New Stress Parameter in Horses in a Stress Model Involving Four Different Stress Levels: D. Scholler, et al.; Animals 13, 1142 (2023), Abstract
A Potential Role for Substance P in West Nile Virus Neuropathogenesis: S.E. Ronca, et al.; Viruses 14, 1961 (2022), Abstract
Aggregation of alpha-synuclein in enteric neurons does not impact function in vitro: Bindas, A. J., Nichols, K. N., et al.; Sci. Rep. 12, 22211 (2022), Abstract
Elevated serum substance P during simian varicella virus infection in rhesus macaques: implications for chronic inflammation and adverse cerebrovascular events: A. Bubak, et al.; J. Neurovirol. 26, 945 (2020), Abstract
Effect of xylazine administration before laparoscopic abomasopexy to correct left displaced abomasum on markers of stress in dairy cows: T.S. Tschoner, et al.; J. Dairy Sci. 103, 9318 (2020), Application(s): Bovine plasma, Abstract
Sensory neuropeptides and their receptors participate in mechano-regulation of murine macrophages: D. Muschter, et al.; Int. J. Mol. Sci. 20, 503 (2019), Application(s): ELISA using culture supernatants, Abstract — Full Text