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Stem cell factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance or survival of hematopoietic stem cells. Human SCF shows no activity on mouse cells, but mouse and rat SCF are active on human cells.
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Figure 1: Rat SCF Bioactivity Data. Serial dilutions of Rat SCF, starting at 250ng/ml, were added to TF-1 cells growing in GM-SCF free media. Cell proliferation was measure after 63 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 for this lot of Rat SCF was between 10-15ng/ml. This value is comparable to the typical expected range of 20-40ng/ml.
Figure 2: 1µg protein under (-) non-reducing in a 4-20% TRIS-Glycine gel, stained with Coomassie Blue. Rat SCF has a predicted MW of 18.4 kDa.
| Regulatory Status |
RUO – Research Use Only |
|---|
Related Products
| Alternative Name | SCF, c-Kit ligand, KL, Steel factor, MGF |
|---|---|
| Purity | ≥97% (SDS-PAGE using Coomassie Staining) |
| Source | Produced in E. coli. Non-glycosylated protein, containing 165 amino acids. |
| Alternative Name | SCF, c-Kit ligand, KL, Steel factor, MGF |
|---|---|
| Purity | ≥95% (reducing and non-reducing SDS-PAGE) |
| Source | Produced in E. coli. Non-glycosylated protein, containing 164 amino acids. |