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Stem cell factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance or survival of hematopoietic stem cells. Human SCF shows no activity on mouse cells, but mouse and rat SCF are active on human cells.
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Figure 1: Mouse SCF Bioactivity Data. Serial dilutions of mouse SCF, starting at 250ng/ml, were added to TF-1 cells growing in GM-SCF free media. Cell proliferation was measure after 65 hours and the linear portion of the curve was us used to calculate the ED50. The ED50 for this lot of mouse SCF was between 34-51ng/ml. The typical range is between 2.5-10ng/ml.
| Regulatory Status |
RUO – Research Use Only |
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| Alternative Name | SCF, c-Kit ligand, KL, Steel factor, MGF |
|---|---|
| Purity | ≥97% (SDS-PAGE using Coomassie Staining) |
| Source | Produced in E. coli. Non-glycosylated protein, containing 165 amino acids. |
| Alternative Name | SCF, c-Kit ligand, KL, Steel factor, MGF |
|---|---|
| Purity | ≥98% (Reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm) |
| Source | Produced in E. coli. Non-glycosylated protein, containing 165 amino acids. |