sPLA₂ activity kit

• Higher throughput – assay up to 42 samples in duplicate per 96 well kit
• Sensitive – detect < 3 U/ml
• Fast – results in just 40 minutes!

The sPLA₂ activity kit is a higher throughput assay for the quantification of up to 42 samples in duplicate per 96 well kit. This kit provides safe, sensitive, and reliable results in under an hour.

Secretory Phospholipase A2s (sPLA₂s) are a group of related enzymes that upon activation, cleave an acyl ester bond in the sn-2 position in glycerophospholipid. These are extracellular enzymes that have high disulfide bond content, low molecular mass (14KDa), and require mM levels of Ca⁺ for catalysis. sPLA₂ enzymes play a crucial role in the generation of arachidonic acid, which is further metabolized to proinflammatory eicosanoids.