Sp-8-Br-2′-O-Me-cAMPS

Sp-8-Br-2’-O-Me-cAMPS is a hydrolysis-resistant form of 8-Br-2’-O-Me-cAMP (Prod. No. BLG-B022) which is a potent membrane-permeant stimulator of the exchange factors directly activated by cAMP (Epac or cAMP-GEF).
Since a free 2’-ribose hydroxyl group in cyclic AMP is essential for stimulation of cAMP-dependent protein kinase (PKA), the methylated structure of Sp-8-Br-2’-O-Me-cAMPS is an extremely poor PKA activator and allows for specific discrimination between both signalling pathways. On the other hand, potent activators of PKA carrying a modified 6 position at the adenine nucleobase can be used as Epac-negative controls. N6-modified cyclic AMP analogues such as N⁶-Benzoyl-cAMP (Prod. No. BLG-B009) or N⁶-Phenyl-cAMP (Prod. No. BLG-P006) are specific PKA agonists, but show only neglectable agonistic properties on Epac.