Sp-8-AHA-cAMPS-Agarose

The protein kinase A activator Sp-cAMPS immobilized on agarose by an aminohexylamino spacer attached to position 8 of the ligand. Free Sp-cAMPS is only very slowly metabolized by corresponding phosphodiesterases and thus represents an extraordinary stable ligand. In addition, its reduced binding affinity towards protein kinase A compared to cAMP should result in milder desorption conditions in affinity chromatography. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others. This ligand is also available with a shorter spacer arm (Sp-8-AEA-cAMPS-Agarose (Prod. No. BLG-A008)).