Sp-8-AET-cGMPS-Agarose

The protein kinase G activator Sp-cGMPS (Prod. No. BLG-G017) immobilized on agarose by an aminoethylthio spacer attached to position 8 of the ligand. Free Sp-cGMPS is only very slowly metabolized and thus represents an extraordinary stable ligand. In addition, its reduced binding affinity towards protein kinase G compared to cGMP should result in milder desorption conditions in affinity chromatography. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinases, phosphodiesterases and others. Gels with the corresponding Rp-isomer (Rp-8-AET-cGMPS-Agarose (Prod. No. BLG-A009)) and the parent cyclic phosphate (8-AET-cGMP-Agarose (Prod. No. BLG-A019)) are also available.