Sp-2′-AHC-cGMPS-Agarose

The PDE-resistant protein kinase G (PKG) activator Sp-cGMPS immobilized on agarose by an aminohexylcarbamoyl spacer attached to the ribose 2′ position of the ligand. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins, especially those which tolerate modification of the 2′ ribose hydroxyl group, such as some phosphodiesterases. This type of gel is also available with immobilized PDE-stable Rp-cGMPS (Rp-2′-AHC-cGMPS-Agarose (Prod. No. BLG-A051)) and with normal cGMP (2′-AHC-cGMP-Agarose (Prod. No. BLG-A059)).