Sp-2′-AHC-cAMPS-Agarose

The PDE-resistant protein kinase A activator Sp-cAMPS immobilized on agarose by an aminohexylcarbamoyl spacer attached to the ribose 2′ position of the ligand. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins, especially those which tolerate modification of the 2′ ribose hydroxyl group such as some phosphodiesterases. This type of gel is also available with immobilized PDE-stable Rp-cAMPS (Rp-2′-AHC-cAMPS-Agarose (Prod. No. BLG-A049)) or with normal cAMP (2′-AHC-cAMP-Agarose (Prod. No. BLG-A058)).