RSSR

ALX-430-102

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Supersensitive spin label

Cell permeable supersensitive spin label (detection limit varies from 10nM to 100nM) for quick detection of reduced thiols using ESR. Allows following the reactions of sulfhydryl groups with RSSR to form thiol spin label adducts, for the monitoring of intracellular redox states of glutathione and other thiols.

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Product Details

Alternative Name	bis-(2,2,5,5-Tetramethyl-3-imidazoline-1-oxyl-4-yl)disulfide, biradical
Appearance	Off-white to yellow crystalline powder.
CAS	123557-49-3
Formula	C₁₄H₂₄N₄O₂S₂
MW	344.5
Purity	≥97% (HPLC, GLC)
Solubility	Soluble in DMSO, water, 100% ethanol or methanol; DMSO is recommended for stock solution.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Protect from light.
Long Term Storage	-20°C
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Negative allosteric modulation of the glucagon receptor by RAMP2: Krishna Kumar, K., O’Brien, E. S., et al.; Cell 186, 1465 (2023), Abstract
Comparative evaluation of spin-label modeling methods for protein structural studies: Tessmer, M. H., Canarie, E. R., et al.; Biophys. J. 121, 3508 (2022), Abstract
Alterations in the Plasma and Red Blood Cell Properties in Patients with Varicose Vein: A Pilot Study: L. Gwozdzinski, et al.; Cardiol. Res. Pract. 2021, 5569961 (2021), Abstract
DEER Spectroscopy Measurements Reveal Multiple Conformations of HIV-1 SOSIP Envelopes that Show Similarities with Envelopes on Native Virions: Stadtmueller, B. M., Bridges, M. D., et al.; Immunity 49, 235 (2018), Abstract
Partially Open HIV-1 Envelope Structures Exhibit Conformational Changes Relevant for Coreceptor Binding and Fusion: Wang, H., Barnes, C. O., et al.; Cell Host Microbe 24, 579 (2018), Abstract
A comparative analysis of multiple sclerosis-relevant anti-inflammatory properties of ethyl pyruvate and dimethyl fumarate: Miljkovic, D., Blaževski, J., et al.; J. Immunol. 194, 2493 (2015), Abstract
Extracellular iron diminishes anticancer effects of vitamin C: an in vitro study: Mojic, M., Bogdanović Pristov, J., et al.; Sci. Rep. 4, 5955 (2014), Abstract
In vitro and in vivo measurement of pH and thiols by EPR-based techniques: V.V. Khramtsov, et al.; Antioxid. Redox Signal. 6, 667 (2004), Abstract
Unique in vivo applications of spin traps: L.J. Berliner, et al.; Free Radic. Biol. Med. 30, 489 (2001), Abstract
Use of imidazoline nitroxides in studies of chemical reactions. ESR measurements of the concentration and reactivity of protons, thiols and nitric oxide.: V.V. Khramtsov & L. B. Volodarsky; Spin labeling. The next Millennium. 145, 109 (1998)

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Last modified: May 29, 2024

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ALX-430-102

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