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rSec8 monoclonal antibody (14G1)

ADI-VAM-SV016

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rSec6 and rSec8 are rat homologs of the yeast Sec6 and Sec8 proteins that are essential for protein transport between the Golgi apparatus and the plasma membrane. In yeast, Sec8 is part of a 19.5S complex that includes Sec6 and Sec15, that also associate with the ras-like GTP binding protein Sec4. An analogous 17S complex in rat brain was found to contain rSec6 and rSec8, along with eight proteins with a combined molecular weight of 743 kDa. The membrane-associated rSec6/8 complex co-immunoprecipitates with syntaxin, providing evidence that this complex is involved in synaptic vesicle docking and fusion at the presynaptic terminal.

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This antibody is covered by our Worry-Free Guarantee.

rSec8 monoclonal antibody (14G1) Western blot — Western blot analysis of rSec8: Lane 1: MWM, Lane 2: Brain (rat), (tissue extract) (Prod No. ADI-LYT-RB100), Lane 3: Brain (mouse), (tissue extract) (Prod No. ADI-LYT-MB100), Lane 4: MDBK Cell Lysate.

rSec8 monoclonal antibody (14G1) Immunohistochemistry — Immunohistochemistry analysis of human brain, cerebellum tissue stained with rSec8, mAb (14G1) at 5µg/ml.

Product Details

Alternative Name	Exocyst complex component 4
Application	ICC, IHC (PS), IP, WB
Application Notes	Detects a band of ~110kDa by Western blot.
Clone	14G1
Formulation	Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
GenBank ID	U32498
Host	Mouse
Immunogen	Recombinant rat rSec8.
Isotype	IgG1
Purity Detail	Protein G affinity purified.
Recommendation Dilutions/Conditions	Immunoprecipitation (12.5µg/ml)Western Blot (1:1,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from ascites.
Species Reactivity	Bovine, Chicken, Dog, Fish, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
UniProt ID	Q62824
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

DLG1 functions upstream of SDCCAG3 and IFT20 to control ciliary targeting of polycystin-2: Rezi, C. K., Aslanyan, M. G., et al.; EMBO Rep. 25, 3040 (2024), Abstract
Exocyst inactivation in urothelial cells disrupts autophagy and activates non-canonical NF-κB signaling: Ortega, M. A., Villiger, R. K., et al.; Dis. Model. Mech. 15, (2022), Abstract
Exocyst Inactivation in Urothelial Cells Disrupts Autophagy and Activates non-canonical NF-κB: Fogelgren, B., Ortega, M. A., et al.; bioRxiv , (2021), Application(s): WB / Reactant(s): Human
The chaperone ERp29 is required for tunneling nanotube formation by stabilizing MSec.: Kumar, R., Bhattacharya, J., et al.; J. Biol. Chem. 294, 7177 (2019), Application(s): WB, Abstract
Primary cilia and the exocyst are linked to urinary extracellular vesicle production and content: Zuo, X., Kwon, S. H., et al.; J. Biol. Chem. 294, 19099 (2019), Abstract
Arl13b and the exocyst interact synergistically in ciliogenesis: C. Seixas, et al.; Mol. Biol. Cell. 27, 308 (2016), Application(s): Immunoblotting of mouse kidney lysates, Abstract — Full Text
PRL-3 disrupts epithelial architecture by altering the post-mitotic midbody position: Lujan, P., Varsano, G., et al.; J. Cell Sci. 129, 4130 (2016), Abstract
Cdc42 and sec10 Are Required for Normal Retinal Development in Zebrafish.: Choi, S. Y., Kim, S. H., et al.; Invest. Ophthalmol. Vis. Sci. 56, 3361 (2015), Application(s): WB / Reactant(s): Zebrafish, Abstract
Characterization of an apical ceramide-enriched compartment regulating ciliogenesis: He, Q., Wang, G., et al.; Mol. Biol. Cell 23, 3156 (2012), Abstract
Proteomic analysis of HIV-1 Nef cellular binding partners reveals a role for exocyst complex proteins in mediating enhancement of intercellular nanotube formation: Mukerji, J., Olivieri, K. C., et al.; Retrovirology 9, 33 (2012), Abstract