Rp-8-CPT-cAMPS

Rp-8-CPT-cAMPS is a membrane permeant, isozyme-selective inhibitor of protein kinase A with special preference for cAK II. Its metabolic stability avoids potential side effects through active metabolites. In contrast to common ATP-site inhibitors, Rp-8-CPT-cAMPS occupies cAMP binding sites at the regulatory subunit of PKA and prevents the kinase holoenzyme from dissociation and thus from activation. Due to this working principle, preincubation of the inhibitor prior to the activation step is necessary for optimal results.