Rp-8-AHA-cAMPS-Agarose

The metabolically stable protein kinase A inhibitor Rp-cAMPS immobilized on agarose by an aminohexylamino spacer attached to position 8 of the ligand. Free Rp-cAMPS binds to the protein kinase A holoenzyme but prevents dissociation to free catalytic and regulatory subunits. In addition, it is not hydrolized by mammalian phosphodiesterases.The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins such as protein kinase A (presumably as its holoenzyme), phosphodiesterases and others. This ligand is also available with a shorter spacer arm (Rp-8-AEA-cAMPS-Agarose (Prod. No. BLG-A007)).