Rp-2′-AEC-cAMPS-Agarose

The protein kinase A inhibitor Rp-cAMPS (Prod. No. BLG-A002) immobilized on agarose by an aminoethylcarbamoyl spacer attached to the ribose 2′-position of the ligand. Free Rp-cAMPS is not metabolized by mammalian phosphodiesterases and thus represents an extraordinary stable ligand. In addition, its reduced binding affinity towards protein kinase A compared to cAMP results in milder desorption conditions during affinity chromatography. The gel can be used for affinity chromatography of various cyclic nucleotide-responsive proteins, especially those which tolerate modification of the 2′-ribose hydroxyl group such as some phosphodiesterases. This type of gel is offered with a longer spacer (Rp-2′-AHC-cAMPS-Agarose, Prod.. No. BLG-A049) as well