- Directly monitors reactive oxygen and/or nitrogen species (ROS/RNS) in live cells
- Discriminates among superoxide, nitric oxide and peroxynitrite
- High sensitivity, specificity and accuracy for live cell studies
- Compatible with major components of tissue culture media (phenol red, FBS and BSA)
- Complete set of reagents, including ROS/RNS inducers and scavengers
Enzo Life Sciences ROS-ID® ROS/RNS detection kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy. The kit includes three detection probes: NO Detection Reagent (Red, Ex/Em 650/670 nm), Oxidative Stress Detection Reagent (Green, Ex/Em 490/525 nm) for total ROS detection, and Superoxide Detection Reagent (Orange, Ex/Em 550/620 nm). It also includes the positive controls, pyocyanin and L-arginine, which are common inducers of ROS and NO production, respectively, as well as the negative controls, N-acetyl-L-cysteine and c-PTIO (common scavengers of ROS and NO, respectively). Through the combination of the three detection probes with a set of specific inhibitors and activators, this kit enables discrimination among superoxide, nitric oxide and peroxynitrite.
Free radicals and other reactive species play seminal roles in many psychological and pathophysiological processes. Once produced within a cell, free radicals can damage a wide variety of cellular constituents, including proteins, lipids and DNA. However, at lower concentrations these very same agents may serve as second messengers in cellular signaling. Information-rich methods are required to quantify the relative levels of various reactive species.
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Regulatory Status |
RUO – Research Use Only |
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