ROS-ID^® NO Detection kit

• Directly monitor real time NO production in live cells by fluorescence microscopy
• High specificity, sensitivity and accuracy, yielding a water-insoluble red fluorescent product
• Distinguish between peroxynitrite and nitric oxide
• NO inducer and scavenger included

Enzo Life Sciences’ ROS-ID^® NO Detection kit is designed to directly monitor real time NO production in live cells by fluorescence microscopy using NO Detection Reagent (red fluorescent) as the major component. The non-fluorescent, cell-permeable NO detection dye reacts with NO in the presence of O₂ with high specificity, sensitivity and accuracy, yielding a water-insoluble red fluorescent product. Importantly, this dye is not reactive towards peroxynitrite that allows distinguishing between peroxynitrite and nitric oxide. The kit is not designed to detect reactive chlorine or bromine species, as the fluorescent probes included are relatively insensitive to these analytes. Upon staining, the fluorescent products generated by this dye can be visualized using a wide-field fluorescence microscope equipped with any red fluorescent cube. The combination of 650/670 nm is recommended when additional fluorescence signals (green or orange) would be detected simultaneously The NO Detection Kit contains sufficient reagents for at least 200 microscopy assays using live cells (adherent or in suspension).