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RIG-I (human) polyclonal antibody (AT111)

ALX-210-932

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RIG-I (retinoic acid-inducible gene I; Ddx58), a member of the DEAD-box RNA helicases, is activated by cytoplasmic dsRNA and 5’-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.

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Product Details

Alternative Name	RIG-1, Retinoic acid-inducible gene 1 protein, DEAD-box protein 58, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 M, Probable ATP-dependent RNA helicase DDX58
Application	IP, WB
Formulation	Liquid. In PBS containing 0.02% sodium azide.
Host	Rabbit
Immunogen	Recombinant human RIG-I (aa 201-713).
Purity Detail	Protein A-affinity purified.
Recommendation Dilutions/Conditions	Immunoprecipitation (1:100)Western blot (1:1,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from rabbit serum.
Species Reactivity	Human
UniProt ID	O95786
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Handling	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Short Term Storage	+4°C
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells: X. Yin, et al.; Cell Rep. 32, 108628 (2021), Application(s): Western Blot, Abstract — Full Text
Structural analysis of RIG-I-like receptors reveals ancient rules of engagement between diverse RNA helicases and TRIM ubiquitin ligases.: Young, J. M., Zhu, Z., et al.; Mol. Cell 81, 599 (2021), Application(s): WB / Reactant(s): Human, Abstract
Structural analysis of RIG-I-like receptors reveals ancient rules of engagement between diverse RNA helicases and TRIM ubiquitin ligases: Kato, K., Ahmad, S., et al.; bioRxiv , (2020)
Ubiquitin-Dependent and -Independent Roles of E3 Ligase RIPLET in Innate Immunity: Cadena, C., Ahmad, S., et al.; Cell 177, 1187 (2019), Abstract
Hepatitis E virus persists in the presence of a type III interferon response: Yin, X., Li, X., et al.; PLoS Pathog. 13, e1006417 (2017), Abstract
Phosphorylation-Dependent Feedback Inhibition of RIG-I by DAPK1 Identified by Kinome-wide siRNA Screening: Willemsen, J., Wicht, O., et al.; Mol. Cell 65, 403 (2017), Abstract
Association of a Network of Interferon-Stimulated Genes with a Locus Encoding a Negative Regulator of Non-conventional IKK Kinases and IFNB1: S. Jeidane, et al.; Cell Rep. 17, 425 (2016), Application(s): Western Immunoblot Analyses, Abstract — Full Text
Retinoic acid-inducible gene-I-like receptor (RLR)-mediated antiviral innate immune responses in the lower respiratory tract: roles of TRAF3 and TRAF5: Y. Chiba, et al.; Biochem. Biophys. Res. Commun. 467, 191 (2015), Application(s): Immunoblot analyses of human tissue, Abstract
ATP hydrolysis by the viral RNA sensor RIG-I prevents unintentional recognition of self-RNA: C. Lässig, et al.; eLife 4, e10859 (2015), Application(s): Dependent signaling, Abstract — Full Text
Activation of an immunoregulatory and antiviral gene expression program in poly(I:C)-transfected human neutrophils: N. Tamassia, et al.; J. Immunol. 181, 6563 (2008), Abstract

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Alternative Name	RIG-1, Retinoic acid-inducible gene 1 protein, DEAD-box protein 58, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 M, Probable ATP-dependent RNA helicase DDX58
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Isotype	IgG2a
Species Reactivity	Mouse

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RIG-I (human) polyclonal antibody (AT111)

ALX-210-932

Product Details

Handling & Storage

Datasheet, Manuals, SDS & CofA

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