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This sequence, AKRRRLSSLRA, derived from rat hepatic 40S ribosomal protein S6 (229-239). Substrate for Rho-kinase II (ROCK II), Km=0.9 µM and for p90 rsk (S6K) in cell extracts (Km=0.18 mM) using phosphocellulose assay. Peptide phosphorylation by PKS and PKC can be eliminated by the inclusion of PKI and EGTA, respectively, in the assay.
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RUO – Research Use Only |
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