Home Shop Raptor monoclonal antibody (10E10)

Raptor monoclonal antibody (10E10)

ADI-905-765

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The mTOR (mammalian target of rapamycin) pathway coordinates nutrient signals with growth factor dependent signaling. mTOR is organized in two independent protein complexes, mTORC1 and mTORC2. The mTORC1 complex containing mTOR, GβL/mLST8 and Raptor is sensitive to rapamycin, while the mTORC2 complex containing mTOR, GβL/mLST8, SIN1, and Rictor is rapamycin insensitive.

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This antibody is covered by our Worry-Free Guarantee.

Raptor monoclonal antibody (10E10) Western blot — Western blot analysis of serum starved cancer cells, probed with Raptor mAb (10E10); HeLa (1); HepG2 (2); HEK293 (3); SH-SY5Y (4); MDCK (5); PC12 (6); CMT- 93 (7); Neuro 2A (8); and NIH-3T3 (9).

Product Details

Application	WB
Application Notes	Detects a band of ~160kDa by Western blot.
Clone	10E10
Formulation	Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
Host	Mouse
Immunogen	Synthetic peptide corresponding to a portion of Raptor.
Isotype	IgG1
Purity Detail	Thiophilic adsorption and size exclusion chromatography purified.
Recommendation Dilutions/Conditions	Western Blot (0.5µg/ml, ECL)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Reconstitution	Reconstitute with 1ml water (15 minutes at room temperature).
Species Reactivity	Dog, Human, Mouse, Rat
UniProt ID	Q8N122
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Use/Stability	Stable at -80°C up to 1 year, at 4°C up to 3 months.
Handling	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Long Term Storage	-20°C
Shipping	Blue Ice