RadioImmunoPrecipitation Assay (RIPA) Buffer, 10X (500 ml)

RIPA buffer is a lysis buffer used to lyse cells and tissues for RadioImmunoPrecipitation assay (RIPA).This buffer in addition to Triton X100 contains ionic detergents SDS and sodium deoxycholate which dissolves nuclear membranes and it also contains EDTA and EGTA. It is a low background producing buffer that can denature kinases. This buffer is not suitable to disrupt protein: protein interaction. Protease inhibitors e.g. PMSF, protease cocktail and phosphatase inhibitors cocktail should be added fresh to RIPA buffer.