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This polyclonal antibody detects RAB7A, a small GTPase involved in endosomal trafficking and autophagy.
RAB7A regulates late endosomal maturation and lysosomal biogenesis. It is essential for autophagy and degradation of cellular waste. Dysregulation of RAB7A has been linked to neurodegeneration and cancer.
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Immunochemical staining of human RAB7A in human kidney with RAB7A polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunofluorescence staining of RAB7A in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit RAB7A polyclonal antibody (dilution ratio 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
| Regulatory Status |
RUO – Research Use Only |
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