pY-SUMOstar Saccharomyces Intracellular Vector

The pY-SUMOstar vector allows for the intracellular expression of SUMOstar fusion proteins in Saccharomyces cerevisiae. SUMOstar fusion constructs are not processed in vivo, allowing for the same enhancement of protein expression, solubility, and stability afforded by SUMOpro.Protein expression is driven by the S. cerevisiae copper metallothionein (CUP1) promoter, which is both tightly regulated and highly inducible.  The plasmid is maintained by the 2-micron element and selected for by the restoration of L-tryptophan biosynthesis in Trp auxotrophs.