[pTyr^239/240]Shc monoclonal antibody (1E3)

Shc transforming protein 1, SH2 domain protein C1, Shc1

ELISA, IHC, WB

1E3

Does not cross-react with non-phosphorylated shc.

Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.

Mouse

Synthetic peptide corresponding to aa 236-243 (D236HQpYpYNDF243) of shc phosphorylated at Tyr239 and Tyr240 conjugated to KLH.

IgG1

Included. (Prod. No. ALX-840-025)

Thiophilic adsorption and size exclusion chromatography purified.

Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Reconstitute with 1ml water (15 minutes at room temperature).

Dog, Human, Mouse, Rat

Recognizes p46shc, p52shc and p66shc when phosphorylated at Tyr^239/240.

P29353

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