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Figure:Lysates of normal lung fibroblasts (WI38) and of human fibrosarcoma (8387) were probed with the PTX3, pAb, (Prod. No. ALX-210-365), over night at +4 °C at a concentration of 500ng/ml in 5% dry milk in PBS+/+ containing 0.05% Tween 20. The signal was revealed with an HRP-conjugated anti-rat IgG secondary antibody in 5% dry milk PBS+/+ containing 0.05% Tween 20 after incubation for 1 hour at room temperature and IMMOBILON Western Chemiluminescent substrate from Millipore.
Figure 2: Pulmonary PTX3 expression by immunohistochemistry (IHC) using PTX3, pAb (Prod. No. ALX-210-365). Method: Photomicrograph of PTX3 stained lung tissue of Cigarette Smoke (CS)-exposed WT mice. Mice were exposed to CS for 4 weeks (subacute).(V: vein, A: artery, AW: airway. Magnification 200×). In courtesy of Guy Brusselle.
| Regulatory Status |
RUO – Research Use Only |
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Related Products
| Alternative Name | Pentraxin 3, TSG14, TNF stimulated gene-14 |
|---|---|
| Source | Produced in CHO cells. Human full-length PTX3. |
| Alternative Name | Pentraxin 3, TSG-14, TNF stimulated gene-14 |
|---|---|
| Application | ELISA, ICC, IHC |
| Host | Rabbit |
| Species Reactivity | Chicken, Human, Mouse, Rat |
| Alternative Name | Pentraxin 3, TSG-14, TNF stimulated gene-14 |
|---|---|
| Application | ELISA, Flow Cytometry, IHC (FS), IP, WB |
| Host | Rat |
| Isotype | IgG2a |
| Species Reactivity | Human, Rat |