Home Shop [pSer82]HSP27 monoclonal antibody (5B9)

[pSer⁸²]HSP27 monoclonal antibody (5B9)

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Alternative Name	Heat shock protein 27
Application	IHC, WB
Clone	5B9
Formulation	Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
GenBank ID	L39370
Host	Mouse
Immunogen	Synthetic peptide corresponding to aa 79-85 (R79QLpSSGV85) of human HSP27 (heat shock protein 27) phosphorylated at Ser82 conjugated to KLH.
Isotype	IgG2a
Positive Control	Included. (Prod. No. ALX-840-031)
Purity Detail	Thiophilic adsorption and size exclusion chromatography purified.
Recommendation Dilutions/Conditions	Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Reconstitution	Reconstitute with 1ml water (15 minutes at room temperature).
Species Reactivity	Dog, Human, Mouse, Rat
Specificity	Recognizes HSP27 phosphorylated at Ser⁸².
Technical Info / Product Notes	Replacement for ADI-905-642
UniProt ID	P04792
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Use/Stability	Upon reconstitution, stable at -80°C for up to 1 year, at +4°C for up to 3 months.
Handling	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Cold water immersion attenuates anabolic signaling and skeletal muscle fiber hypertrophy, but not strength gain, following whole-body resistance training: Fyfe, J. J., Broatch, J. R., et al.; J. Appl. Physiol. 127, 1403 (2019), Abstract
Small heat shock proteins translocate to the cytoskeleton in human skeletal muscle following eccentric exercise independently of phosphorylation.: Murphy, R. M., Lamb, G. D., et al.; J. Appl. Physiol. 116, 1463 (2014), Reactant(s): Human, Abstract
In vitro and in vivo therapeutic approach for a small cell carcinoma of the ovary hypercalcaemic type using a SCCOHT-1 cellular model: Otte, A., Rauprich, F., et al.; Orphanet. J. Rare Dis. 9, 126 (2014), Abstract
The mechanisms of the widespread production of phosphorylated HSP25 after fatiguing muscle stimulation: Y. Jammes, et al.; J. Exp. Biol. 216, 3620 (2013), Application(s): Use as detection antibody in ELISA, Abstract — Full Text

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