[pSer326]HSF1 ELISA kit

• Sensitive – measure pg/ml of HSF1 and use less precious sample compared to Western blot
• Time Saving – results from up to 40 samples in duplicate in just 3 hours
• Convenient – ready-to-use liquid color-coded reagents and pre-coated plate

The HSF1 EIA kit is a complete kit for the quantitative determination of HSF1 phosphorylated at Ser³²⁶ in cell lysates and tissue extracts from human and mouse origins.

HSF1 belongs to a family of heat shock transcription factors (HSFs) that bind to highly conserved heat shock elements (HSEs) in the promoter regions of heat shock genes and regulate the expression of heat shock proteins (Hsps). Most HSFs have several common functional motifs including an N-terminal DNA binding domain essential for binding to the HSE and adjacent hydrophobic repeats essential for the formation of active trimers. Another short hydrophobic repeat located towards the C-terminal region of most HSFs is thought to be necessary for suppression of trimerization except in the case of yeast HSF and human HSF4. In higher eukaryotes, HSF1 is predominantly found in a diffuse cytoplasmic and nuclear distribution in unstressed cells. On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleo-plasmic distribution. HSF1 is post-translationally modified by both phosphorylation and sumoylation. Inducible phosphorylation at Ser230, Ser326, and Ser419 promote HSF1 activity, while constitutive phosphorylation sites at Ser303, Ser307, and Ser308 are inhibitory.