[pSer²³⁹]VASP monoclonal antibody (16C2) (biotin conjugate)

Vasodilator stimulated phosphoprotein

ELISA, Flow Cytometry, ICC, IP, WB

16C2

Does not cross-react with the non-phosphorylated form of VASP.

Liquid. 0.5mg/ml in 2x PBS containing 0.09% sodium azide, PEG and sucrose.

Mouse

Synthetic peptide corresponding to a portion of VASP (vasodilator stimulated phosphoprotein) phosphorylated at Ser239 (RKVpSKQE) conjugated to KLH.

IgG1

Thiophilic adsorption and size exclusion chromatography purified.

ELISA (use at 0.05µg/ml)Immunocytochemistry (use at 1-10µg/ml; may tolerate 0.5% formaldehyde fixation)Immunoprecipitation (use 1-10 µg per 106 pervanadate-treated A431 cells)Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Human, Mouse, Rabbit, Rat

Recognizes Ser²³⁹-phosphorylated VASP.

Important! The use of MAb to VASP (activated) (Ser²³⁹) (16C2) for diagnostic or therapeutic purposes is patented! The antibody is supplied for research use only!

P50552

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