[pSer²³⁹]VASP monoclonal antibody (16C2)

Vasodilator stimulated phosphoprotein

ELISA, Flow Cytometry, ICC, IP, WB

16C2

Does not cross-react with the non-phosphorylated form of VASP.

Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.

Mouse

Synthetic peptide corresponding to a portion of VASP (vasodilator stimulated phosphoprotein) phosphorylated at Ser239 (RKVpSKQE) conjugated to KLH.

IgG1

Thiophilic adsorption and size exclusion chromatography purified.

ELISA (use at 0.05µg/ml)Immunocytochemistry (use at 1-10µg/ml; may tolerate 0.5% formaldehyde fixation) , Immunoprecipitation (use 1-10 µg per 106 pervanadate-treated A431 cells)Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Reconstitute with 1ml water (15 minutes at room temperature).

Human, Mouse, Rabbit, Rat

Recognizes Ser²³⁹-phosphorylated VASP.

Important! The use of MAb to VASP (activated) (Ser²³⁹) (16C2) for diagnostic or therapeutic purposes is patented! The antibody is supplied for research use only!

P50552

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