Home Shop [pSer157]VASP monoclonal antibody (5C6)

[pSer¹⁵⁷]VASP monoclonal antibody (5C6)

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Alternative Name	Vasodilator-stimulated phosphoprotein
Application	ELISA, FUNC, ICC, IP, WB
Application Notes	Functional Application: monitoring of PKA activity.
Clone	5C6
Crossreactivity	Does not cross-react with the non-phosphorylated form of VASP.
Formulation	Lyophilized from 1ml of PBS containing 0.09% sodium azide, PEG, and sucrose.
Host	Mouse
Immunogen	Synthetic peptide corresponding to a portion of human VASP (vasodilator-stimulated phosphoprotein) phosphorylated at Ser157 conjugated to KLH.
Isotype	IgG1
Purity Detail	Thiophilic adsorption and size exclusion chromatography purified.
Recommendation Dilutions/Conditions	Immunocytochemistry (1-10µg/ml; may tolerate 0.5% formaldehyde fixation)Western Blot> (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Reconstitution	Reconstitute with 1ml water (15 minutes at room temperature).
Species Reactivity	Human, Mouse
Technical Info / Product Notes	The use of MAb to VASP (activated) (Ser¹⁵⁷) (5C6) for diagnostic or therapeutic purposes is patented! The antibody is supplied for research use only! Replacement for ADI-905-768
UniProt ID	P50552
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Use/Stability	Stable at -80°C up to 1 year, at 4°C up to 3 months.
Handling	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

A conjugate of decyltriphenylphosphonium with plastoquinone can carry cyclic adenosine monophosphate, but not cyclic guanosine monophosphate, across artificial and natural membranes: A.M. Firsov, et al.; Biochim. Biophys. Acta 1860, 329 (2018), Application(s): Western Blot, Abstract
Regulation of human endothelial cell focal adhesion sites and migration by cGMP-dependent protein kinase I: A. Smolenski, et al.; J. Biol. Chem. 275, 25723 (2000), Abstract — Full Text
KT5823 inhibits cGMP-dependent protein kinase activity in vitro but not in intact human platelets and rat mesangial cells: M. Burkhardt, et al.; J. Biol. Chem. 275, 33536 (2000), Abstract — Full Text

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