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Protein carbonyl ELISA kit

ALX-850-312

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High sensitivity ELISA for quantitative determination of carbonylated protein levels in plasma, other body fluids, cell and tissue extracts.

High sensitivity ELISA, detecting low levels of carbonylated proteins
Validated in various sample types
Fully quantitative results that surpass semi-quantitative Western blot analysis

The Protein carbonyl ELISA kit is an antibody-based detection set. It has higher sensitivity, lower background, is less labour-intensive and handles more samples per day as compared to colorimetric assays.

Protein carbonyls are formed by a variety of oxidative mechanisms and are sensitive indices of oxidative injury. The quantity of protein carbonyls in a protein sample can be determined by derivatising with dinitrophenylhydrazine (DNP) and measure bound DNP colorimetrically or immunologically. The ELISA method enables carbonyls to be measured quantitatively with microgram quantities of protein.

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Protein carbonyl ELISA kit Standard Curve — Standard curve of Protein carbonyl ELISA kit

Product Details

Application	ELISA
Application Notes	For quantitative determination of carbonylated protein levels in plasma, other body fluids, cell and tissue extracts.
Assay Time	4.5 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Quantity	96 wells (~80 tests)
Species Reactivity	Species independent
Wavelength	450 nm

Handling & Storage

Use/Stability	Remove box containing components G, K, and L and store at -20°C! Remove box containing components H and I and store at 4°C! DO NOT FREEZE! Store the remaining of the kit at room temperature. The kit is stable at room temperature for up to 6 weeks.
Handling	Do not freeze.
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

Serum oxidative biomarkers associated with genital HPV infection and cervical lesions in women: Lin, H. Y., Zhu, X., et al.; J. Med. Virol. 96, e29362 (2024), Abstract
Oxidative stress induction by narasin augments doxorubicin’s efficacy in osteosarcoma: Z. Han, et al.; BMC Pharmacol. Toxicol. 24, 56 (2023), Abstract
Repurposing Ivermectin to augment chemotherapy’s efficacy in osteosarcoma: B. Hu, et al.; Hum. Exp. Toxicol. 41, 9.60327E+15 (2022), Abstract
Preliminary experience on the use of sucrosomial iron in hemodialysis: focus on safety, hemoglobin maintenance and oxidative stress: F. Reggiani, et al.; Int. Urol. Nephrol. 10, 11255 (2021), Abstract
Evaluation of Thiol-dependent Enzymes on the Pharmacological Effects Induced by the Catalytically Active PLA2 from Bothrops jararacussu: Toyama, M. H., Costa, C. R. C., et al.; Preprints.org , (2021)
Cardiovascular and Blood Oxidative Stress Responses to Exercise and Acute Woodsmoke Exposure in Recreationally Active Individuals: C.M. Williamson-Reisdorph, et al.; Wilderness Environ. Med. , (2021), Abstract
Oxygen Exposure During Cardiopulmonary Resuscitation Is Associated With Cerebral Oxidative Injury in a Randomized, Blinded, Controlled, Preclinical Trial: Marquez, A. M., Morgan, R. W., et al.; J. Am. Heart Assoc. 9, e015032 (2020), Abstract
Identification of Toxicity Parameters Associated with Combustion Produced Soot Surface Chemistry and Particle Structure by in Vitro Assays: Al Housseiny, H., Singh, M., et al.; Biomedicines 8, (2020), Abstract
Plasma protein carbonylation in haemodialysed patients: focus on diabetes and gender: G. Colombo, et al.; Oxid. Med. Cell. Longev. 2018, 4149681 (2018), Application(s): ELISA using human plasma, Abstract — Full Text
Antibiotic drug piperacillin induces neuron cell death through mitochondrial dysfunction and oxidative damage: S. Jiang, et al.; Can. J. Physiol. Pharmacol. 96, 562 (2018), Application(s): SH-SY5Y cell lysates, Abstract