Protein Carbonyl Assay

Oxidative stress due to an imbalance between ROS production and antioxidant defense leads to cellular damage including the oxidation of amino acid side chains, resulting in protein carbonyls. Protein carbonylation disrupts the function and structure of proteins involved in various metabolic pathways. Protein carbonylation triggers immune responses, leading to chronic inflammation affecting things like insulin sensitivity and lipid metabolism. Elevated levels of protein carbonyls are associated with age-related diseases like neurodegenerative disorders, cardiovascular disease, and diabetes. AkrivisBio’s Protein Carbonyl Assay is a simple sensitive means of determining protein carbonyls in a variety of samples with a sensitivity well below 1 nmole.