Home Shop Proteasome 20S β5 subunit (human) polyclonal antibody

Proteasome 20S β5 subunit (human) polyclonal antibody

BML-PW8895

56 citations

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BML-PW8895 Proteasome 20S β5 subunit (human) polyclonal antibody Western Blot 1 — Human cancer cell lines may be grouped according to the basal enzyme activities and expression levels of APEH and proteasome.Cells from eight human cancer lines (U87, MCF7, MDA-MB, HeLa, Caco2, HepG2, A375M, A375) and non-cancerous cells (BHk21, fibroblasts) were harvested at the pre-confluent stage and used for cytoplasmic or mRNA extract preparation. Basal APEH and proteasomal CT-like activities were measured in cytoplasmic extracts (A). The mRNA levels of APEH and β-5 subunit were evaluated by qRT-PCR and expressed as fold change in comparison to expressed levels in human fibroblast (B). Intracellular levels of β-5 and APEH were detected by immunoblotting (C upper panel). Typical Western blot was shown and data from three different analyses were normalized to the density of control protein (β-actin) and expressed as ratio over control (C lower panel). Results are presented as the mean values ±SD of triplicate analyses from at least three different experiments. *Significantly different (P < 0.01) from respective controls.

Image collected and cropped by CiteAb under a CC-BY license from the following publication: RedOx status, proteasome and APEH: insights into anticancer mechanisms of t10,c12-conjugated linoleic acid isomer on A375 melanoma cells. PLoS One (2013)

Product Details

Alternative Name	Proteasome subunit β type-5, Macropain ε chain, Proteasome chain 6, Proteasome subunit MB1, Proteasome subunit X
Application	IHC, WB
Formulation	Liquid. In PBS containing 0.09% sodium azide.
Gene/Protein Identifier	PSMB5 (gene name)
Host	Rabbit
Immunogen	Synthetic peptide corresponding to aa 194-207 of human proteasome 20S (β5 subunit).
Source	Purified from rabbit serum.
Species Reactivity	Human
Specificity	Recognizes human proteasome 20S β5 subunit.
Technical Info / Product Notes	Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from “standard” nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins.
UniProt ID	P28074
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Handling	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Loss-of-function mutations in the dystonia gene THAP1 impair proteasome function by inhibiting PSMB5 expression.: Ramage, D. E., Grant, D. W., et al.; Nat. Commun. 16, 1511 (2025), Reactant(s): Human, Abstract
The nociceptive activity of peripheral sensory neurons is modulated by the neuronal membrane proteasome: E.V. Landero, et al.; Cell Rep. 43, 114058 (2024), Abstract
Oleuropein enhances proteasomal activity and reduces mutant huntingtin-induced cytotoxicity.: Huang, Z. N., Lee, S. Y., et al.; Front. Pharmacol. 15, 1459909 (2024), Application(s): WB / Reactant(s): Mouse, Abstract
Investigating the Expression and the Activity Patterns of Proteasomal Subunits in Livers with HBV infection and HBV-Derived HCC: Januzzi, A. T., Sari, G., et al.; Research Square , (2024)
Loss-of-function mutations in the dystonia gene THAP1 impair proteasome function by inhibiting PSMB5 expression: Ramage, D. E., Grant, D. W., et al.; bioRxiv , (2024), Reactant(s): Human
An engineered cell line with a hRpn1-attached handle to isolate proteasomes.: Negi, H., Osei-Amponsa, V., et al.; J. Biol. Chem. 299, 104948 (2023), Application(s): WB, Abstract
Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction.: Kim, M. J., Serwa, R. A., et al.; Nat. Commun. 14, 4092 (2023), Application(s): WB / Reactant(s): Human, Abstract
Design, synthesis, and biological evaluation of some novel naphthoquinone-glycine/β-alanine anilide derivatives as noncovalent proteasome inhibitors: Tatar, I., Uysal, S., et al.; Chem. Biol. Drug Des. 101, 1283 (2023), Abstract
The role of Cycloastragenol at the intersection of Nrf-2/ARE, telomerase, and proteasome activity: Kırmızıbayrak, P. B., Yılmaz, S., et al.; bioRxiv , (2022), Reactant(s): Human
Healthspan improvement and anti-aggregation effects induced by a marine-derived structural proteasome activator: M.A. Vasilopoulou, et al.; Redox Biol. 56, 10262 (2022), Abstract

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Proteasome 20S β5 subunit (human) polyclonal antibody

BML-PW8895

Product Details

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