Home Shop Proteasome 20S β2 subunit monoclonal antibody (MCP165)

Proteasome 20S β2 subunit monoclonal antibody (MCP165)

BML-PW9300

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The hybridoma secreting the antibody to subunit β2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunization with dinitrophenylated proteasomes. The antibody (clone MCP165) has been characterised by one- and two dimensional Western blotting. The antibody has not been demonstrated to immunoprecipitate the native 20S proteasome. The immunoglobulin subclass is IgG₁.

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Proteasome 20S β2 subunit monoclonal antibody (MCP165) Western blot — Western Blot Analysis of Proteasome 20S β2 subunit, mAb (MCP165), (Prod. No. BML-PW9300): Lane 1: MW Marker, Lane 2: HeLa Cell Lysate (Prod. No. ADI-LYC-HL100)

Product Details

Alternative Name	Proteasome subunit β type-7, Macropain chain Z
Application	IHC, WB
Application Notes	Immunoprecipitation: Does not immunoprecipitate intact proteasomes from HeLa cell preparations. Immunohistochemistry: Has been shown to have applicability in immunohistochemical applications using human tissue. Western blot: Single dimension SDS-PAGE of a HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of ~28kDa.
Clone	MCP165
Formulation	Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Gene/Protein Identifier	PSMB7 (gene name)
Host	Mouse
Immunogen	Dinitrophenylated proteasomes.
Isotype	IgG1
Purity Detail	Protein G affinity purified.
Recommendation Dilutions/Conditions	Western Blot (1:1,000)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.
Source	Purified from hybridoma tissue culture supernatant.
Species Reactivity	Human, Mouse
Specificity	Recognizes the β2 subunit of the 20S proteasome.
Technical Info / Product Notes	Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from “standard” nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins.
UniProt ID	Q99436 (human), P70195 (mouse)
Worry-free Guarantee	This antibody is covered by our Worry-Free Guarantee.

Handling & Storage

Use/Stability	Store unopened vial at -20°C until required for use. Aliquot undiluted antibody into smaller volumes (not less than 10μl) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month.
Handling	Avoid freeze/thaw cycles.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Genome-wide siRNA screens identify RBBP9 function as a potential target in Fanconi anaemia-deficient head-and-neck squamous cell carcinoma.: Pai, G., Roohollahi, K., et al.; Commun. Biol. 6, 37 (2023), Application(s): WB / Reactant(s): Human, Abstract
Orthogonal approaches required to measure proteasome composition and activity in mammalian brain tissue: Turker, F., Bharadwaj, R. A., et al.; J. Biol. Chem. 299, 104811 (2023), Abstract
Antioxidant Molecular Brain Changes Parallel Adaptive Cardiovascular Response to Forced Running in Mice.: Bartra, C., Jager, L. A., et al.; Antioxidants (Basel) 11, (2022), Application(s): WB / Reactant(s): Mouse, Abstract
Resveratrol confers neuroprotection against high-fat diet in a mouse model of Alzheimer’s disease via modulation of proteolytic mechanisms: Sarroca, S., Gatius, A., et al.; J. Nutr. Biochem. 89, 108569 (2021), Abstract
An SOD1 deficiency aggravates proteasome inhibitor bortezomib-induced testicular damage in mice: T. Homma & J. Fujii; Biochim. Biophys. Acta Gen. Subj. 1863, 1108 (2019), Application(s): Mouse cryosectioned tissue, Abstract
Heterozygous Truncating Variants in POMP Escape Nonsense-Mediated Decay and Cause a Unique Immune Dysregulatory Syndrome.: Lupski, J. R., Gibbs, R. A., et al.; Am. J. Hum. Genet. 102, 1126 (2018), Application(s): WB / Reactant(s): Human, Abstract
Potential involvement of ubiquitin-proteasome system dysfunction associated with oxidative stress in the pathogenesis of sickle cell disease: Warang, P., Homma, T., et al.; Br. J. Haematol. 182, 559 (2018), Abstract
Major Histocompatibility Complex (MHC) Class I Processing of the NY-ESO-1 Antigen Is Regulated by Rpn10 and Rpn13 Proteins and Immunoproteasomes following Non-lysine Ubiquitination: Golnik, R., Lehmann, A., et al.; J. Biol. Chem. 291, 8805 (2016), Abstract
Protein Expression of Proteasome Subunits in Elderly Patients with Schizophrenia: Scott, M. R., Rubio, M. D., et al.; Neuropsychopharmacology 41, 896 (2016), Abstract
SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes: T. Homma, et al.; Arch. Biochem. Biophys. 583, 65 (2015), Application(s): Immunoblotting, Abstract