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Product Details
| Alternative Name |
PROS30, Proteasome subunit α type-1, Macropain subunit C2, Proteasome ν chain |
|---|---|
| Application |
IHC, IP, WB |
| Application Notes |
Immunoprecipitation: This antibody has been immobilised on CNBr-activated Sepharose CL-4B and used to precipitate proteasomes from HeLa cells. Proteasomal subunits were subsequently separated by 2D PAGE. |
| Clone |
MCP20 |
| Crossreactivity |
Does not cross-react with rat or mouse protein. |
| Formulation |
Liquid. In PBS containing 10mM sodium azide. |
| Gene/Protein Identifier |
PSMA1 (gene name) |
| Host |
Mouse |
| Immunogen |
Human placenta derived proteasomes. |
| Isotype |
IgG1 |
| Purity Detail |
Protein G affinity purified. |
| Source |
Purified from ascites. |
| Species Reactivity |
Human, Rabbit |
| Specificity |
Recognizes the α6 subunit of proteasome 20S. |
| Technical Info / Product Notes |
Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from “standard” nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins. |
| UniProt ID |
P25786 |
| Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
| Use/Stability |
Aliquot undiluted antibody into smaller volumes (not less than 10µL) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-mouse IgG linker antibody is to be used). |
|---|---|
| Handling |
Avoid freeze/thaw cycles. |
| Long Term Storage |
-20°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Inhibition of nucleo-cytoplasmic proteasome translocation by the aromatic amino acids or silencing Sestrin3-their sensing mediator-is tumor suppressive: Livneh, I., Fabre, B., et al.; Cell Death Differ. 31, 1242 (2024), Abstract
- DDI2 protease controls embryonic development and inflammation via TCF11/NRF1: Nedomova, M., Haberecht-Müller, S., et al.; iScience 27, 110893 (2024), Abstract
- Targeted degradation via direct 26S proteasome recruitment: Bashore, C., Prakash, S., et al.; Nat. Chem. Biol. 19, 55 (2023), Application(s): WB, Abstract
- The role of Cycloastragenol at the intersection of Nrf-2/ARE, telomerase, and proteasome activity: Kırmızıbayrak, P. B., Yılmaz, S., et al.; bioRxiv , (2022), Reactant(s): Human
- The role of cycloastragenol at the intersection of NRF2/ARE, telomerase, and proteasome activity.: Yılmaz, S., Bedir, E., et al.; Free Radic. Biol. Med. 188, 105 (2022), Application(s): ChIP / Reactant(s): Human, Abstract
- Functional and quantitative evaluation of the 20S proteasome in serum and intracellular in145 moroccan patients with hematologic malignancies: H. Filali, et al.; Clin. Proteomics 19, 41 (2022), Abstract
- Proteasome Subunits Differentially Control Myeloma Cell Viability and Proteasome Inhibitor Sensitivity.: Stewart, W., Shi, C. X., et al.; Mol. Cancer Res. 18, 1453 (2020), Application(s): WB / Reactant(s): Human, Abstract
- Structural insights into loss of function of a pore forming toxin and its role in pneumococcal adaptation to an intracellular lifestyle: Badgujar, D. C., Anil, A., et al.; PLoS Pathog. 16, e1009016 (2020), Abstract
- Novel proteasome assembly chaperone mutations in PSMG2/PAC2 cause the autoinflammatory interferonopathy CANDLE/PRAAS4: de Jesus, A. A., Brehm, A., et al.; J. Allergy Clin. Immunol. 143, 1939 (2019), Abstract
- Heterozygous Truncating Variants in POMP Escape Nonsense-Mediated Decay and Cause a Unique Immune Dysregulatory Syndrome.: Lupski, J. R., Gibbs, R. A., et al.; Am. J. Hum. Genet. 102, 1126 (2018), Application(s): WB / Reactant(s): Human, Abstract
- NEDDylation promotes nuclear protein aggregation and protects the Ubiquitin Proteasome System upon proteotoxic stress.: Maghames, C. M., Lobato-Gil, S., et al.; Nat. Commun. 9, 4376 (2018), Application(s): WB / Reactant(s): Human, Abstract
- Inhibition of calpain-1 stabilizes TCF11/Nrf1 but does not affect its activation in response to proteasome inhibition: Nowak, K., Taubert, R. M., et al.; Biosci. Rep. 38, (2018), Abstract
- Proteasome activation enhances stemness and lifespan of human mesenchymal stem cells: M. Kapetanou, et al.; Free Radic. Biol. Med. 103, 226 (2017), Abstract
- Reduction of RPT6/S8 (a Proteasome Component) and Proteasome Activity in the Cortex is Associated with Cognitive Impairment in Lewy Body Dementia: Alghamdi, A., Vallortigara, J., et al.; J. Alzheimers Dis. 57, 373 (2017), Abstract
- The proteasome maturation protein POMP increases proteasome assembly and activity in psoriatic lesional skin: B.A. Zieba, et al.; J. Dermatol. Sci. 88, 10 (2017), Reactant(s) Human, Abstract
- Major Histocompatibility Complex (MHC) Class I Processing of the NY-ESO-1 Antigen Is Regulated by Rpn10 and Rpn13 Proteins and Immunoproteasomes following Non-lysine Ubiquitination: Golnik, R., Lehmann, A., et al.; J. Biol. Chem. 291, 8805 (2016), Abstract
- Circulating 20S Proteasome Is Independently Associated with Abdominal Muscle Mass in Hemodialysis Patients: H. Fukasawa, et al.; PLoS One 10, e0121352 (2015), Application(s): Western Blot, Abstract — Full Text
- Direct cellular delivery of human proteasomes to delay tau aggregation: Han, D. H., Na, H. K., et al.; Nat. Commun. 5, 5633 (2014), Abstract
- Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE): D.S. Pitcher, et al.; Biochim. Biophys. Acta 1844, 2222 (2014), Application(s): WB / Reactant(s) E. coli, Abstract
- TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions: Z. Lukic, et al.; Retrovirology 8, 93 (2011), Abstract — Full Text
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