Home Shop Proteasome 19S Rpn10/S5a subunit (human), (recombinant) (GST-tag) (agarose immobilized)

Proteasome 19S Rpn10/S5a subunit (human), (recombinant) (GST-tag) (agarose immobilized)

BML-UW8635

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Manual
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Rpn10/S5a, a subunit of the 19S regulator of the 26S proteasome, binds multi-ubiquitinylated proteins containing chains of at least four ubiquitin moieties. Rpn10/S5a-agarose retains this ability and may be used to identify and purify multi-ubiquitinylated proteins.

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Product Details

Alternative Name	26S proteasome non-ATPase regulatory subunit 4, Antisecretory factor 1, Multiubiquitin chain-binding protein
Formulation	Liquid. The resin is supplied as a slurry in 50mM TRIS, pH 7.5, containing 150mM sodium chloride, 50% glycerol, 1mM DTT and 0.01% sodium azide.
Purity	≥95% (SDS-PAGE)
Source	GST-S5a (human Rpn10) is covalently coupled to agarose at a concentration of 0.5mg protein/ml of settled resin.
UniProt ID	P55036

Handling & Storage

Handling	Do not freeze.
Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Activity Dependent Protein Degradation Is Critical for the Formation and Stability of Fear Memory in the Amygdala: T.J. Jarome, et al.; PLoS One 6, e24349 (2011), Abstract
Hyperubiquitination of proteins in dilated cardiomyopathy: J. Weekes, et al.; Proteomics 3, 208 (2003), Abstract
Purification of poly-ubiquitinated proteins by S5a-affinity chromatography: R. Layfield, et al.; Proteomics 1, 773 (2001), Abstract

Datasheet, Manuals, SDS & CofA

Manuals And Inserts

Datasheet

Specific Protocol

Human S5a sequence, with an N-terminal fusion of glutathione S-transferase (GST) (Schistosoma japonicum), was expressed in E. coli. After purification and analysis the expressed protein was coupled to CNBr activated-agarose and unbound proteins removed by washing. The loading of protein is determined spectrophotometrically during preparation and is shown to be approximately 0.5mg protein per ml of settled resin.

For use, it is recommended that polyubiquitinylated proteins are bound in TBS or PBS and, after extensive washing, eluted by heating for 5 minutes at 95ºC in Laemmli loading buffer prior to SDS-PAGE/Western blot analysis. Alternatively polyubiquitinylated proteins can be eluted using buffer containing 7M urea.

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