Proteasome 19S Rpn10/S5a subunit (human), (recombinant) (GST-tag)

A subunit of the 26S proteasome, S5a, recognises and binds multi-ubiquitinylated proteins containing chains of at least four ubiquitin moieties. Purified recombinant S5a retains the ability to bind multi-ubiquitinylated proteins in isolation, when immobilised on glutathione-Sepharose, and after being eluted from glutathione-Sepharose, run on SDS-PAGE, and blotted onto nitrocellulose. Human S5a sequence, with an N-terminal fusion of glutathione S-transferase (Schistosoma japonicum), was expressed in E. coli with GST tag at amino terminus.

S5a (RPN10), a subunit of the 19S regulator of the 26S proteasome, binds multi-ubiquitinylated proteins containing chains of at least four ubiquitin moieties.