Procathepsin V (human), (recombinant) (His-tag)

Cathepsin L2

Useful tool to study enzyme kinetics, cleave target substrates and screen for inhibitors.

Liquid. In 25mM TRIS-HCl, pH 8.0, containing 100mM sodium chloride, 0.05% Tween-20 and 10% glycerol.

~37kDa (SDS-PAGE)

≥90%

Produced in insect cells. Recombinant glycosylated procathepsin V cloned from human cDNA and purified as full-length proenzyme. Produced in a baculovirus expression system.

436 U/µg protein. One unit will hydrolyze one pmole Z-Leu-Arg-AMC substrate (Prod. No. BML-P229, 25 µM) per minute at 25°C, in 25mM NaOAc pH 5.5, 100mM NaCl, 1.0mM DTT.

The proenzyme can be activated as in Adachi et al. (Reference year 2008): Dilute proenzyme into 200mM NaAcetate, pH 6.0, with 0.05% SDS, 2.5mM DTT, and incubate at 37°C for 5-30 minutes. Alternatively, pre-incubate proenzyme 5-30 minutes in assay buffer (25mM NaOAc pH 5.5, 100mM sodium chloride, 1.0mM DTT), then add substrate to begin assay. Incubation times must be determined empirically; activation is dependent on factors such as buffer, temperature, and enzyme concentration, and cathepsin V will autodegrade once activated.

O60911