PRL-1 (human), (recombinant) (His-tag)

Phosphatase of regenerating liver 1, Protein tyrosine phosphatase type IVA 1

Useful for studies of enzyme kinetics and regulation, dephosphorylation of target substrates, and inhibitor screening.

Liquid. In 40mM TRIS-HCl, pH 8.0, containing 110mM sodium chloride, potassium chloride, 200mM imidazole, 20% glycerol and 3mM DTT.

~19.8kDa

≥75% (SDS-PAGE)

Produced in E. coli. The catalytic domain of PRL-1 (aa 2-173) is fused at the N-terminus to a His-tag.

≥0.168U/min/μg. One unit will hydrolyze 1pmol para-nitrophenyl phosphate (PNPP) per minute at 37°C. Assay buffer: 50mM TRIS, pH 7.4, containing 150mM sodium chloride, 5mM dithiothreitol and 12.5mM PNPP.

Q93096