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PPH . hydrochloride

ALX-430-080

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Spin trapping reagent

Effective, non-cell permeable and non-toxic analog of CPH for the detection of superoxide radicals. Resistant against reduction of vitamin C. For cell permeable analog see CPH (Prod. No. ALX-430-078).

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Product Details

Alternative Name	1-Hydroxy-4-phosphono-oxy-2,2,6,6-tetramethylpiperidine . HCl
Appearance	White to off-white solid.
Formula	C₉H₂₀NO₅P . HCl
MW	253.2 . 36.5
Purity	≥99% (EPR)
Purity Detail	≤0.1% paramagnetic impurities (EPR)
Solubility	Soluble in DMSO, 100% ethanol, methanol or water.
Technical Info / Product Notes	The stabilized acid form of PPH is not well soluble at neutral pH (~0.1-0.01%). It is recommended to adjust pH to 7.4 for higher solubility. Dissolve PPH in oxygen-free buffers at neutral pH and use 1M NaOH to adjust pH to 7.4. Solubility is 1% in water, 0.8% in methanol or 0.5% in ethanol at pH 7.4. Solubility is 3% in DMSO at pH 7.4 (first add NaOH to PPH crystals then DMSO). Higher concentrations require further pH adjustment using NaOH.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Long Term Storage	-20°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

EPR detection of cellular and mitochondrial superoxide using cyclic hydroxylamines: S.I. Dikalov, et al.; Free Rad. Res. 45, 417 (2011), Abstract
Molecular Mechanisms of Angiotensin II Mediated Mitochondrial Dysfunction. Linking Mitochondrial Oxidative Damage and Vascular Endothelial Dysfunction: A.K. Doughan, et al.; Circ. Res. 102, 488 (2008), Abstract
Rotenone model of Parkinson disease: multiple brain mitochondria dysfunctions after short term systemic rotenone intoxication: A. Panov, et al.; J. Biol. Chem. 280, 42026 (2005), Abstract
Noninvasive diagnostic tool for inflammation-induced oxidative stress using electron spin resonance spectroscopy and an extracellular cyclic hydroxylamine: S.I. Dikalov, et al.; Arch. Biochem. Biophys. 402, 218 (2002), Abstract
A new approach for extracellular spin trapping of nitroglycerin-induced superoxide radicals both in vitro and in vivo: B. Fink, et al.; Free Radic. Biol. Med. 28, 121 (2000), Abstract
Detection of superoxide radicals and peroxynitrite by 1-hydroxy-4- phosphonooxy-2,2,6,6-tetramethylpiperidine: quantification of extracellular superoxide radicals formation: S. Dikalov, et al.; BBRC 248, 211 (1998), Abstract

Related Products

CPH . hydrochloride

ALX-430-078

Spin probe reagent

14 citations

Alternative Name	1-Hydroxy-3-carboxy-2,2,5,5-tetramethylpyrrolidine . HCl
CAS	164364-89-0
Purity	≥99% (EPR)

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PPH . hydrochloride

ALX-430-080

Product Details

Handling & Storage

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