POLYVIEW^® PLUS HRP-DAB (Anti-Mouse) Kit

IHC, ISH (in situ hybridization)

< 2 hours

POLYVIEW^® PLUS AP (Anti-Rabbit) Reagent (Ready-To-Use) (ENZ-ACC110), HIGHDEF^® Red AP Chromogen Kit (ENZ-ACC158), HIGHDEF^® Hematoxylin (ENZ-ACC106), Antigen Retrieval Reagent, pH 9 (10X) (ENZ-ACC113), DIGX^® Rabbit anti-digoxigenin linker (ENZ-ABS303), Proteinase K (ENZ-33801), AMPIVIEW™ Wash Buffer 1, AMPIVIEW™ Wash Buffer 2

The POLYVIEW^® PLUS HRP-DAB (Anti-Mouse) Kit contains POLYVIEW^®  PLUS HRP (Anti-Mouse) Reagent that has been developed by directly labeling anti-mouse immunoglobulins with enzymes using a proprietary tandem hyper-labeling technology in ready-to-use format. This ensures consistent and reproducible immunodetection of mouse antibodies against nuclear, cytoplasmic and membrane antigens in different types of tissues. The single step POLYVIEW^® PLUS HRP (Anti-Mouse) Reagent allows faster staining procedures as compared to traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background. Enzo’s HIGHDEF^® DAB (Brown) HRP and HIGHDEF^® Hematoxylin reagents provide strong signal coupled with the peroxidase and antibody blocking blockers included in the kit to give minimal background.