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POLYVIEW^®PLUS HRP (anti-mouse) reagent

ENZ-ACC104

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High sensitivity, low background nanopolymer detection reagent for use with HIGHDEF^® chromogens in ISH and IHC applications

Ready-to-use reagent
Biotin-free nanopolymer detection circumvents endogenous biotin background
High intensity color development delivers sharp, crisp staining
Compatible with both automated and manual platforms
The Antibody Blocker/Diluent further reduces background (sold separately)
Customize with HIGHDEF^® chromogens of your choice (sold separately)

The POLYVIEW^®PLUS HRP (anti-mouse) reagent is a ready-to-use, biotin-free, one-step detection reagent suitable for immunohistochemical detection of antigens in formalin-fixed paraffin-embedded tissues and frozen sections. The detection reagent may also be used with blood smears, cytosmears, and cell preparations. In addition, the POLYVIEW^®PLUS HRP (anti-mouse) reagent can be adapted for in situ hybridization detection.

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POLYVIEW PLUS HRP (anti-mouse) reagent Immunohistochemistry Mousebrain b-amyloid-p-tau — Dual IHC staining performed on formalin-fixed paraffin-embedded tissue section from human brain with Alzheimer’s disease. The tissue sections were stained for alpha-amyloid with POLYVIEW® PLUS AP (anti-rabbit) reagent (Prod. No. ENZ-ACC110) and HIGHDEF® Green AP Chromogen/Substrate (Prod. No. ENZ-ACC130), and phospho-tau with POLYVIEW® PLUS HRP (anti-mouse) reagent (Prod. No. ENZ-ACC104) and HIGHDEF DAB Chromogen/Substrate Set (Prod. No. ENZ-ACC105). Picture courtesy of Dr. Karelle Leroy Laboratoire d’histologie, de neuroanatomie et de neuropathologie, Université Libre de Bruxelles, Bruxelles, Belgium.

POLYVIEW PLUS HRP (anti-mouse) reagent Immunohistochemistry tonsil tissue — Tonsil tissue stained with anti-Ki-67 antibody (mouse), followed by POLYVIEW^® PLUS HRP Reagent

POLYVIEW® PLUS HRP (anti-mouse) reagent ISH-HRS-POLYVIEW-PLUS-anti-mouse

POLYVIEW® PLUS HRP (anti-mouse) reagent Immunohistochemistry — Cervical tissue stained with anti-Ki-67 antibody (mouse), followed by POLYVIEW^® PLUS HRP (anti-mouse) reagent.

POLYVIEW® PLUS HRP (anti-mouse) reagent Immunohistochemistry ADbrain b-amyloid-p-tau — Dual IHC staining performed on formalin-fixed paraffin-embedded tissue section from transgenic mouse brain expressing human mutated APP and PS1. The tissue sections were stained for alpha-amyloid with POLYVIEW® PLUS AP (anti-rabbit) reagent (Prod. No. ENZ-ACC110) and HIGHDEF® Green AP Chromogen/Substrate (Prod. No. ENZ-ACC130), and phospho-tau with POLYVIEW® PLUS HRP (anti-mouse) reagent (Prod. No. ENZ-ACC104) and HIGHDEF DAB Chromogen/Substrate Set (Prod. No. ENZ-ACC105). Picture courtesy of Dr. Karelle Leroy Laboratoire d’histologie, de neuroanatomie et de neuropathologie, Université Libre de Bruxelles, Bruxelles, Belgium.

Product Details

Application	IHC, ISH (in situ hybridization)
Technical Info / Product Notes	The POLYVIEW^®PLUS HRP (anti-mouse) reagent has been developed by directly labeling anti-mouse immunoglobulins with a nanopolymer of horseradish peroxidase (HRP) using proprietary technology. This ensures consistent and reproducible immunodetection of mouse primary antibodies against nuclear, cytoplasmic and membrane antigens in different types of tissues. The single step methodology enables faster staining procedures than traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background. The POLYVIEW^®PLUS HRP (anti-mouse) reagent is suitable for use with mouse IgG antibodies. The reagent can be used for manual staining or with automated staining instruments and are well suited for multiplex immunohistochemical staining assays. The Antibody Blocker/Diluent (ENZ-ACC108) further reduces background and is suitable for diluting the POLYVIEW^®PLUS HRP (anti-mouse) reagent.

Application

IHC, ISH (in situ hybridization)

Technical Info / Product Notes

The POLYVIEW^®PLUS HRP (anti-mouse) reagent has been developed by directly labeling anti-mouse immunoglobulins with a nanopolymer of horseradish peroxidase (HRP) using proprietary technology. This ensures consistent and reproducible immunodetection of mouse primary antibodies against nuclear, cytoplasmic and membrane antigens in different types of tissues. The single step methodology enables faster staining procedures than traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background.

The POLYVIEW^®PLUS HRP (anti-mouse) reagent is suitable for use with mouse IgG antibodies. The reagent can be used for manual staining or with automated staining instruments and are well suited for multiplex immunohistochemical staining assays.

The Antibody Blocker/Diluent (ENZ-ACC108) further reduces background and is suitable for diluting the POLYVIEW^®PLUS HRP (anti-mouse) reagent.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended.
Handling	Do not freeze.
Long Term Storage	+4°C
Shipping	Blue Ice

Regulatory Status	RUO – Research Use Only

Inhibition of DNMT3B and PI3K/AKT/mTOR and ERK Pathways as a Novel Mechanism of Volasertib on Hypomethylating Agent-Resistant Cells: E.J. Choi, et al.; Biomol. Ther. 31, 319 (2023), Abstract
Functional plasticity and recurrent cell states of malignant B cells in follicular lymphoma: Attaf, N., Dong, C., et al.; bioRxiv , (2022), Application(s): IHC / Reactant(s): Human
Elevated Expression of MiR-17 in Microglia of Alzheimer’s Disease Patients Abrogates Autophagy-Mediated Amyloid-b Degradation: S. Estfanous, et al.; Front. Immunol. 12, 705581 (2021), Abstract
The CCR5 antagonist maraviroc causes remission of pancreatic cancer liver metastasis in nude rats based on cell cycle inhibition and apoptosis induction: H. Huang, et al.; Cancer Lett. 474, 82 (2020), Abstract
Diagnostic pathology of Alzheimer’s disease from routine microscopy to immunohistochemistry and experimental correlations: G. Nuovo, et al.; Ann. Diagn. Pathol. 28, 24 (2017), Abstract
False-positive results in diagnostic immunohistochemistry are related to horseradish peroxidase conjugates in commercially available assays: G. Nuovo; Ann. Diagn. Pathol. 25, 54 (2016), Application(s): Compare secondary HRP conjugate from Leica, Ventana, and Enzo in side-by-side comparrison on the Leica BOND-MAX, Abstract