Home Shop Polyinosinic-polycytidylic acid . potassium salt (TLRGRADE®) (synthetic)

Polyinosinic-polycytidylic acid . potassium salt (TLRGRADE^®) (synthetic)

ALX-746-021

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Activator of TLR3 and MDA5

Specific activator of TLR3 and MDA5
Used to generate stable mature dendritic cells
Potential adjuvant in cancer vaccines

TLR3 is a key effector of the immune response against viruses by recognizing double-stranded RNA. Polyinosinic-polycytidylic acid (Poly(I:C)), a synthetic double-stranded homopolymer, binds TLR3 and activates the transcription factor interferon regulator factor 3 (IRF3) following the initiation of TIR domain-containing adaptor protein (TRIF)-dependent TLR signaling. Activation of IRF3 by poly(I:C) leads to the production of type I interferons. Poly(I:C) also activates the RNA helicases MDA-5 and RIG-1. Poly(I:C) can be used to generate stable mature dendritic cells in vitro and is considered a potent adjuvant in vaccine formulations, especially those targeting dendritic cells, thanks to the synergy between MDA5 and TLR3 activation.

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Polyinosinic-polycytidylic acid . potassium salt (TLRGRADE) (synthetic) image — **Figure 2:** Poly(I:C) . K (TLRGRADE®) (synthetic) (Prod. No. ALX-746-021) induces cytokine release in mouse *in vivo*. **Method:** Poly(I:C) was injected i.v. and plasma levels of IFN α/β were determined 2 hours later by cytokine ELISA.

Product Details

Alternative Name	poly(I:C) . K, poly(I:C)
CAS	31852-29-6
Couple Target	MDA, TLR, TLR3
Couple Type	Activator, Ligand
Endotoxin Content	<0.002EU/µg (LAL test; BioWhittaker)
Formula	(C₁₀H₁₀N₄NaO₇P)_x • (C₉H₁₁N₃NaO₇P)_x
Formulation	Lyophilized. Sterile.
Purity Detail	Activity and endotoxin tested – TLRGRADE®.
Quantity	Aliquoted by weight of dry material. Contains polymer, residual salt and water. Sufficient for at least 200 cellular activation assays at 10-25µg/ml.
Reconstitution	For a 1mg/ml stock solution, dissolve the total vial content in 2ml (ALX-746-021-M002) or 5ml (ALX-746-021-M005) endotoxin-free buffer or water. To obtain optimal dissolving we recommend the following procedure: – Add 50% of the solvent and let dissolve for 10 min. – Add remaining 50% of the solvent and mix thoroughly. – Moderate warming may aid dissolving.
Source	Synthetic.

Handling & Storage

Use/Stability	As indicated on product label or CoA when stored as recommended. Aqueous stock solution is stable for 1 day when stored at +4°C.
Handling	Protect from light. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C.
Long Term Storage	+4°C
Shipping	Ambient Temperature

Regulatory Status	RUO – Research Use Only

Functional and dynamic profiling of transcript isoforms reveals essential roles of alternative splicing in interferon response: Ueda, M. T., Inamo, J., et al.; Cell Genom. 4, 100654 (2024), Abstract
Synthetic circular RNA switches and circuits that control protein expression in mammalian cells: S. Kameda, et al.; Nucleic Acids Res. 51, e24 (2023), Abstract
Nicotinamide mononucleotide (NMN) alleviates the poly(I:C)-induced inflammatory response in human primary cell cultures: H. Sano, et al.; Sci. Rep. 13, 11765 (2023), Abstract
CCL5-producing migratory dendritic cells guide CCR5+ monocytes into the draining lymph nodes: K. Rawat, et al.; J. Exp. Med. 220, e20222129 (2023), Abstract
Function conservation and disparities of zebrafish and human LGP2 genes in fish and mammalian cells responsive to poly (I: C): X. Gong, et al.; Front. Immunol. 13, 985792 (2022), Abstract — Full Text
N4BP1 negatively regulates NF-κB by binding and inhibiting NEMO oligomerization: H. Shi, et al.; Nat. Commun. 12, 1379 (2021), Abstract
Role of extracellular microRNA-146a-5p in host innate immunity and bacterial sepsis: S. Wang, et al.; iScience 24, 103441 (2021), Abstract — Full Text
IRF-8/miR-451a regulates M-MDSC differentiation via the AMPK/mTOR signal pathway during lupus development: G. Shi, et al.; Cell Death Discov. 7, 41420 (2021), Abstract
Time-Lapse Imaging of Necroptosis and DAMP Release at Single-Cell Resolution: S. Murai , et al.; Methods Mol. Biol. 2274, 353 (2021), Abstract
Human cDC1s express in-doleamine 2, 3-dioxygenase 1 (IDO) with functional effects on T cell priming: S.P. Sittig, et al.; Eur. J. Immunol. 51, 1494 (2021), Abstract