PO₄-AM₃

Phosphate tris(acetoxymethyl)ester

148334-61-6

Substrate

C₉H₁₅O₁₀P

314.2

> 95% (MS)

Due to its rather high lipophilicity, the solubility of PO4-AM3 in water or buffers is limited. We suggest to use a small amount of anhydrous organic solvent such as anhydrous DMSO or DMF for preparation of 1-100mM stock solutions, and to dilute with water or buffer down to the concentrations required. In some cases, especially at high concentrations (~ 1mM), Pluronic® F-127 (Molecular Probes) can be useful to facilitate solubilization in water or physiological media. Please be sure to check for DMSO/DMF tolerance in your system. Since PO4-AM3 is bioactivated by esterases, application to cell cultures should be performed without serum supplements (even heat-inactivated serum still contains active esterases!) in the media for at least 15 minutes. Otherwise, serum esterases may strongly reduce the cell-loading efficacy. Rinse tube walls carefully and preferably use ultrasonic or vortex to achieve total and uniform mixing.

For the Original Manufacturer’s data sheet please click here.