pM-SUMOstar Mammalian Secretory Vector

The pM-secSUMOstar vector allows for expression and secretion of SUMO fusion constructs in a mammalian cell system.  Expression is driven by the strong constitutive human cytomegalovirus (CMV) promoter and efficiently terminated by the bovine growth hormone (BGH) polyadenylation sequence.  Proteins are directed to the secretory pathway by the mouse IgG kappa secretory signal, which precedes a His6 tag. SUMOstar fusions can be expressed transiently or as stable chromosomal integrants in a wide variety of cell lines.  Expression can be further enhanced by episomal replication in SV40 infected cell lines or those that express the SV40 large T antigen (i.e. HEK-293T).