pM-SUMOstar Mammalian Intracellular Vector

The pM-SUMOstar vector allows for cytosolic expression of SUMO fusion constructs in a mammalian cell system.  Expression is driven by the strong constitutive human cytomegalovirus (CMV) promoter and efficiently terminated by the bovine growth hormone (BGH) polyadenylation sequence.  SUMO fusions can be expressed transiently or as stable chromosomal integrants in a wide variety of cell lines.  Expression can be further enhanced by episomal replication in SV40 infected cell lines or those that express the SV40 large T antigen (i.e. HEK-293T).