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PKC kinase activity kit

ADI-EKS-420A

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Non-radioactive protein kinase activity assay provides rapid and sensitive detection

Non-radioactive measurement of kinase activity
Flexible format, kinetic and end-point options available
Fast results in < 4.5 hours
Efficiency maximized with only 30 µl diluted sample needed per well

The PKC Kinase activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.

Protein Kinase C (PKC) is a large superfamily of serine/threonine kinases that mediate essential cellular signals required for activation, proliferation, differentiation and survival. There are at least ten PKC isotypes that are closely related in structure but that have distinct patterns of tissue distribution and function. The PKC isotypes can be subdivided into three classes based on primary structure and biochemical properties. These are: classical or conventional PKC isotypes (cPKC), novel PKC isotypes (nPKC) and atypical PKC isotypes (aPKC).

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Product Details

Alternative Name	Protein kinase C
Application	Activity assay, Colorimetric detection
Application Notes	For the measurement of PKC kinase activity partially purified, purified, or crude enzyme preparations from any species. Cited sample type includes tissue (ref. 1).
Assay Time	4.5 hours
Compatibility	This product is compatible with the Absorbance 96 Plate Reader.
Contents	Microtiter plate, Antibody, Conjugate, Antibody dilution buffer, Kinase assay dilution buffer, ATP, Active Kinase, Wash buffer concentrate, TMB Substrate, Stop solution 2
Species Reactivity	Species independent

Handling & Storage

Use/Stability	Store all components at 4°C, except active kinase at -80°C.
Shipping	Dry Ice

Regulatory Status	RUO – Research Use Only

Reversing cardiac hypertrophy and heart failure using a cardiac targeting peptide linked to miRNA106a: Lu, M., Cai, S., et al.; Clin. Transl. Med. 15, e70432 (2025), Abstract
High-mobility group protein B1 derived mutant peptide mB Box-97 inhibits the formation of neutrophil extracellular traps: More, K. R., Devaraj, A., et al.; Front. Immunol. 16, 1565252 (2025), Abstract
IDH status dictates oHSV mediated metabolic reprogramming affecting anti-tumor immunity: Sahu, U., Mullarkey, M. P., et al.; Nat. Commun. 16, 3874 (2025), Abstract
Glucocorticoids paradoxically promote steroid resistance in B cell acute lymphoblastic leukemia through CXCR4/PLC signaling: Abdoul-Azize, S., Hami, R., et al.; Nat. Commun. 15, 4557 (2024), Abstract
Amyloid β oligomers enhance presynaptic exocytosis via CaV2.1 to drive disease progression in Alzheimer’s models: Jeans, A., Padamsey, Z., et al.; Research Square , (2024)
Regulatory Mechanism on Anti-Glycolytic and Anti-Metastatic Activities Induced by Strobilanthes crispus in Breast Cancer, In Vitro: S.N.H. Muhammad, et al.; Pharmaceuticals (Basel) 16, 153 (2023), Abstract
PKC-βII is downregulated in the premature ovarian failure SD rat model: V. Ubba, et al.; Reprod. Biol. 23, 100754 (2023), Abstract
Extracellular vesicles derived from CD4+ T cells carry DGKK to promote sepsis-induced lung injury by regulating oxidative stress and inflammation: G.W. Tu, et al.; Cell. Mol. Biol. Lett. 28, 24 (2023), Abstract
Bioactive fractions from Allium humile alleviate the risk of high fat diet induced atherosclerosis in albino Wistar rats by inhibiting protein kinase C: S. Anees, et al.; Fitoterapia 172, 105775 (2023), Abstract
Acyl-CoA: diacylglycerol acyltransferase 1 inhibition in the small intestine increases plasma transaminase activity via the activation of protein kinase C pathway: H. Yokoyama, et al.; J. Toxicol. Sci. 19, 47 (2022), Abstract

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Sensitivity	Non-acetylated: 0.49 pmol/ml (assay buffer) / 1.18 pmol/ml (HCl) (range (non-acetylated) 0.78 – 200 pmol/ml)Acetlylated: 0.027 pmol/ml (assay buffer) / 0.006 pmol/ml (HCl) (range (acetylated) 0.078 – 20 pmol/ml)

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PKC kinase activity kit

ADI-EKS-420A

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