Phosphotyrosine monoclonal antibody (16F4)

ELISA, IP, WB

16F4

Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.

Mouse

Phosphotyrosine containing peptides conjugated to KLH.

IgG1

Included. (Prod. No. 850-051-KI02)

Thiophilic adsorption and size exclusion chromatography purified.

Western Blot (0.5µg/ml for HRPO/ECL detection; recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.

Reconstitute with 1ml water (15 minutes at room temperature).

Species independent

Epitope: …DIpYAE…Recognizes phosphotyrosine in the context of the surrounding amino acids, tolerating hydrophobic amino acids directly neighbouring the phosphotyrosine.

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